Protocols for Accelerated Breeding Scheme (ABS)

A information page about protocols.

Module 1. Procedures and Crossing Techniques for CIP's Potato Breeding Program

Module 2. Procedures for Generation of Potato Tuber Families from true (Botanical) Seed

Potato breeding relies on the generation of new genetic combinations through sexual reproduction achieved by pollinating flowers to produce berries which contain botanical seeds. To distinguish botanical seed from vegetative tuber seed, the product of sexual reproduction is also called true seed (TS).

Module 3. Producing Seed Tuber

The potato Clones have to be distributed across regions as in vitro plantlets with a health status that can guarantee that pathogens are not carried by the planting material. Within region potato clones may be disseminated as “healthy seed tuber”, however the quarantine restrictions within each region have to be followed.

Module 4. Healthy Tuber Yield Trials

Healthy Tuber Yield Trials can be conducted with 1 up to 30 clones and is recommended for advanced materials that have already shown superior performance in intentional exposure trials for key traits.

Module 5. Methods for Inoculation and Evaluation of Potato Clones for Virus Resistance

Module 6. Field Assessment of Resistance in Potato to Phytophtora Infestans

Late blight, caused by the oomycete Phytophthora infestans (Mont.) de Bary, is the main biotic constraint to potato production worldwide. The present guide is designed to assist professionals and technicians in charge of evaluation trials designed to screen selected potato genotypes for resistance to this disease.

Module 7. Tuber Bulking Maturity Assessment

Breeders at the Genetic and Crop Improvement Global Program (GCI) know that breeding clones must be suited to the cropping systems and length of the potato growing season of a particular region within their agro-ecological area of adaptation. In this sense, tuber bulking information of clones is of great interest for recommending testing toward final adoption.

Module 8. Abiotic Stress Protocols

This module aims to aide in the design of drought and salinity tolerance experiments to understand the genetics of phenological, physiological, and biochemical traits of potatoes, using quantitative methods to reduce subjectivity, and to increase appropriateness. Therefore, this reference point could facilitate the comparison of results across different experiments regionally and globally as well as allow for better interpretation of results.

Module 9. Assessment of Dormancy and Sprouting Behavior of Elite and Advanced Clones

The present protocol is formulated to support the assessment and documentation of the dormancy period, sprout growth and weight loss of CIP’s elite and advanced potato clones. This information will guide farmers to manipulate sprouting to occur only when it is desirable.

Module 10. Participatory Varietal Selection (PVS) - Mother and Baby Trial Design

This guide offers a precise description of how to carry out the evaluation at the time of flowering, harvest, and postharvest. It contains some recommendations pertaining to the methods to be applied. If we want to compare data from different environments, it is advisable that standardized methods exist for the collection of data.

Module 11. Protocol for assessing bacterial wilt resistance in greenhouse and field conditions

Bacterial wilt, caused by Ralstonia solanacearum (Yabuuchi et al., 1995) is the second most important potato disease in tropical and sub-tropical regions of the world after late blight (Champoiseau et al., 2010).

Module 12. Protocol for Assessment of Post-Harvest Traits

The primary objective of evaluating post-harvest traits is to obtain information about the potential or aptitude of intermediate and advanced clones for diverse end-uses, ranging from fresh consumption to processed products.